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Lab Report 1 Allison M. Shaw Bio 302L Sec: 009 Thursday February 12th, 2014 Introduction: HL-60 cells were initially taken from a Caucasian female that had acute promyelocytic leukemia (Cirtain, Mittelstadt, Higgins, Pollson, 2002). These cells are good for studying cellular processes and under favorable conditions, are â€Å"immortal†. HL-60 cells have a relatively quick doubling time of no more than 48 hours, and do not experience apoptosis, which makes them â€Å"immortal† (Cirtain, et. al, 2002). The HL-60 cell line’s immortality is instrumental when it comes to finding possible treatments for patients with leukemia, which is of interest to researchers. This immortality attribute is also critical for studying mechanisms of monocyte and granulocyte differentiation (Collins, 1987). Another quality about the HL-60 cell line that is equally important is its ability to differentiate into the white blood cell line (Collins, 1987). Granulocytes and monocytes are the two most important types of differentiated cells in this experiment, although white blood cells also differentiate into macrophage-like cells and eosinophils (Collins, 1987). Compounds occurring naturally that induce HL-60 cell line differentiation include Vitamin D (Collins, 1987). Chemical inducers, such as DMSO and PMA in this experiment, can induce HL-60 cells to differentiate into cells along the myeloid path (Cirtain et al,. 2002). DMSO is studied as a control for PMA treatment conditions and is used to make PMA solution. It causes HL-60 cells to differentiate mostly into granulocytes (Cirtain et al,. 2002). The control in this experiment is cells treated with DMSO, and is done to show DMSO has no effect on PMA (Cirtain et al,. 2002). Using kinase C, PMA activates cert... ...he line. Further studies could allow researchers to gain valuable information possibly leading to cures for numerous cancer types. References: Cirtain, Mittelstadt, Higgins, Pollson. â€Å"Chapter 3.† Power Point. Cell and Molecular Biology Laboratory Manual. Great River Tech. Web. 8 Feb 2014. Cirtain, Mittelstadt, Higgins, Pollson. â€Å"Chapter 4.† Power Point. Cell and Molecular Biology Laboratory Manual. Great River Tech. Web. 8 Feb 2014. Collins, S. J. "The HL-60 Promyelocytic Leukemia Cell Line: Proliferation, Differentiation, and Cellular Oncogene Expression." The Blood Journal 70 (1987): 1233-1244. Xie, Bei, Amale Laouar, and Eliezer Huberman. "Fibronectin-mediated Cell Adhesion Is Required for Induction of 92-kDa Type IV Collagenase/Gelatinase (MMP-9) Gene Expression during Macrophage Differentiation." The Journal of Biological Chemistry (1998): 1576-1582.